patent on oxymetholone

[liquid_c]

Syntex was the company to come out with Anadrol.  They were bought up by Roche a while back.

[Vet]

hes an expert, watch your mouth convict 

Shit, candy is showing just how much he doesn't know in this thread. 

[Vet]

Procedure— Proceed as directed for Procedure under 'Single-steroid Assay 511' (see below), using a solvent system consisting of a mixture of benzene and alcohol (98:2), through the fourth sentence of the second paragraph under Procedure. Then centrifuge the tubes for 5 minutes, and determine the absorbances of the supernatants in 1-cm cells at the wavelength of maximum absorbance at about 315 nm, with a suitable spectrophotometer, against the blank. [NOTE—Use 0.01 N alcoholic sodium hydroxide, rather than alcohol, to elute the silica gel bands.] Calculate the quantity, in mg, of C21H32O3 in the portion of Oxymetholone taken by the formula:
10C(AU / AS), in which C is the concentration, in mg per mL, of USP Oxymetholone RS (see below) in the Standard preparation, and AU and AS are the absorbances of the solutions from the Assay preparation and the Standard preparation, respectively.


single-steroid assay 511 - In the following procedure, the steroid to be assayed is separated from related foreign steroids and excipients by thin-layer chromatography and determined following recovery from the chromatogram.

Preparation of the Plate— Prepare a slurry from 30 g of chromatographic silica gel with a suitable fluorescing substance by the gradual addition, with mixing, of about 65 mL of a mixture of water and alcohol (5:2). Transfer the slurry to a clean, 20- × 20-cm plate, spread to make a uniform layer 250 µm thick, and allow to dry at room temperature for 15 minutes. Heat the plate at 105 for 1 hour, and store in a desiccator.

Solvent A— Mix methylene chloride with methanol (180:16).

Solvent B— Mix chloroform with acetone (4:1).

Standard Preparation— Dissolve in a mixture of equal volumes of chloroform and alcohol a suitable quantity of the USP Reference Standard specified in the individual monograph, previously dried as directed (see USP Reference Standards 11) and accurately weighed, to obtain a solution having a known concentration of about 2 mg per mL.

Assay Preparation— Prepare as directed in the individual monograph.

Procedure— Divide the area of the chromatographic plate into three equal sections, the left and right sections to be used for the Assay Preparation and the Standard Preparation, respectively, and the center section for the blank. Apply 200 µL each of the Assay Preparation and the Standard Preparation as streaks 2.5 cm from the bottom of the appropriate section of the plate. Dry the solution as it is being applied, with the aid of a stream of air. Using the Solvent specified in the individual monograph, develop the chromatogram in a suitable chamber, previously equilibrated and lined with absorbent paper, until the solvent front has moved 15 cm above the initial streaks.
Remove the plate, evaporate the solvent, and locate the principal band occupied by the Standard Preparation by viewing under UV light. Mark this band, as well as corresponding bands in the Assay Preparation and blank sections of the plate. Remove the silica gel from each band separately, either by scraping onto glazed weighing papers or by using a suitable vacuum collecting device, and transfer it to a glass-stoppered, 50-mL centrifuge tube. To each tube add 25.0 mL of alcohol, and shake for not less than 2 minutes. Centrifuge the tubes for 5 minutes, pipet 20 mL of the supernatant from each tube into a glass-stoppered, 50-mL conical flask, add 2.0 mL of a solution prepared by dissolving 50 mg of blue tetrazolium in 10 mL of methanol, and mix. Proceed as directed for Procedure under Assay for Steroids 351, beginning with “Then to each flask.”

USP Oxymetholone RS— Dry portion in vacuum over phosphorus pentoxide for 4 hours before using. Keep container tightly closed.

is this what i'm after ?

To me, this reads as a portion of the lab proceedure to identify oxymetholone.   It also reads as if there are parts missing.   The last paragraph is somewhat disjointed too.  Do you have the rest?  Procedure under Assay for Steroids 351?   

[WillGrant]

no thats incorrect. how i explained it to you is accurate.

hahaha funny bastard. 

[Exal]

Vet at first I thought that too, but without the last part it's kinda hard to know, cause what they are doing could be used to make it I guess, just a very wierd way to do it imo... I'm not really into steroid production

[benz]

Shit, candy is showing just how much he doesn't know in this thread. 

no doubt vet, no doubt. Be sure to save this thread in your harddrive, such opportunities to learn come once in a year and its free! 

hahaha funny bastard. 

[Fatpanda]

To me, this reads as a portion of the lab proceedure to identify oxymetholone.   It also reads as if there are parts missing.   The last paragraph is somewhat disjointed too.  Do you have the rest?  Procedure under Assay for Steroids 351?   

i thought that too at first, its very difficult to work out. thats why i was after the patent. 

heres the steroid assay 351 - The following procedure is applicable for determination of those Pharmacopeial steroids that possess reducing functional groups such as -ketols.

Standard Preparation— Dissolve in alcohol a suitable quantity of the USP Reference Standard specified in the individual monograph, previously dried under the conditions specified in the individual monograph and accurately weighed, and dilute quantitatively and stepwise with alcohol to obtain a solution having a concentration of about 10 µg per mL. Pipet 20 mL of this solution into a glass-stoppered, 50-mL conical flask.

Assay Preparation— Prepare as directed in the individual monograph.

Procedure— To each of the two flasks containing the Assay Preparation and the Standard Preparation, respectively, and to a similar flask containing 20.0 mL of alcohol to serve as the blank, add 2.0 mL of a solution prepared by dissolving 50 mg of blue tetrazolium in 10 mL of methanol, and mix. Then to each flask add 2.0 mL of a mixture of alcohol and tetramethylammonium hydroxide TS (9:1), mix, and allow to stand in the dark for 90 minutes. Without delay, concomitantly determine the absorbances of the solutions from the Assay Preparation and the Standard Preparation at about 525 nm, with a suitable spectrophotometer, against the blank. Calculate the result by the formula given in the individual monograph, in which C is the concentration, in µg per mL, of the Reference Standard in the Standard Preparation; and AU and AS are the absorbances of the solutions from the Assay Preparation and the Standard Preparation, respectively.

[Exal]

thats definately just a identification procedure then

[Fatpanda]

thats definately just a identification procedure then

  damn.

i'll have to keep looking then. 

[Vet]

thats definately just a identification procedure then

I agree.  And if it was a manufacturing proceedure, the yield would be ridicoulously small.