Getbig Bodybuilding, Figure and Fitness Forums
Getbig Main Boards => Gossip & Opinions => Topic started by: Sir Bigness on July 21, 2009, 12:01:51 AM
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Should an amateur come off totally when not compeating to save receptors or is there a low dosage for a certain period of time before trying to put on size again?
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Should an amateur come off totally when not compeating to save receptors or is there a low dosage for a certain period of time before trying to put on size again?
That depends on what you mean by "compeating"
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Should an amateur come off totally when not compeating to save receptors or is there a low dosage for a certain period of time before trying to put on size again?
"""HI FERLLAS,,,IN THE LIFE WHEN YOU WANT SOME.THING YOU MUST DO SCACRIFISE,,,COME OFF AND YOU 4LL STAY A LOCAL AMATEUR SO IF YOU WANT TO SAVE RECEPTOR JUST SWITCH WITH OTHER ROID.Z JUST SWITCH FROM TEST TO TREN OR GRAPEFRUIT"""
GH15 APPROVED
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receptor downgrade is a fairy tale!!!!!
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someone please save my receptor
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receptor downgrade is a fairy tale!!!!!
then why isn't the 70yr old juicers as big as the 25yr old?
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"""HI FERLLAS,,,IN THE LIFE WHEN YOU WANT SOME.THING YOU MUST DO SCACRIFISE,,,COME OFF AND YOU 4LL STAY A LOCAL AMATEUR SO IF YOU WANT TO SAVE RECEPTOR JUST SWITCH WITH OTHER ROID.Z JUST SWITCH FROM TEST TO TREN OR GRAPEFRUIT"""
GH15 APPROVED
good point.
If you want success in BB, just up the dosage and juice on!
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here is my question !!!
say you use 1g of test a week.
then you want to reduce it for a few weeks.. lets say for 10 weeks you drop it to 500mg
will you still make gain ? Or it won't do shit to your body since you used more before
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then why isn't the 70yr old juicers as big as the 25yr old?
Because his receptor got old and lazy?
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here is my question !!!
say you use 1g of test a week.
then you want to reduce it for a few weeks.. lets say for 10 weeks you drop it to 500mg
will you still make gain ? Or it won't do shit to your body since you used more before
that would interest me as well.
I heard from a few guys that never used more steroids and still made good gains.
Wo soll ich anfangen....wie viele wissen trainiere ich bereits seit 14 Jahren, Stoff seit gut 10 Jahren. Seit diesem Jahr nun habe ich erhebliche Probleme mit dem Blutdruck bekommen - Gründe lagen unter anderem an der Dauereinnahme von Ephe und sicher auch den Roids. Also habe ich erstmal Ephe raus gelassen, der Blutdruck kam somit schonmal auf ein erträgliches Niveau.
Da ich beruflich derzeit sehr eingespannt bin und auch nur noch unregelmäßig trainieren kann (mal morgens, mal abends, und wenn dann höchstens 3 x die Woche), habe ich die Roids auch heruntergeschraubt - vor allem auch weil ich auf einer Ami Seite etwas sehr interessantes gelesen hatte, was den Stack aus Tren, Testo P und Stanozolol betrifft. Ich fahre nun seit 8 Wochen diesen Stack, in (für meine Verhältnisse) ULTRA GERINGEN Dosis:
50mg Testo P eod (Montag, Mittwoch, Freitag)
0,5ml TriTren (BD) eod
10mg Stano ed
Wie mann sieht, komme ich so auf 150mg testo P, 300mg TrenMix (Acetat, Hexa, Enanthate) und 70mg Stano PRO WOCHE.
Mit diesem Stack komme ich seit guten 8 Wochen extrem gut zurecht, habe wirklich weder Muskelmasse noch Kraft verloren, ich finde sogar das ich eher noch Fortschritte gemacht habe. Für mich verblüffend, bedenkt mann was ich vorher schon alles an Mengen usw. genommen habe.
Ich wollte das nur kurz berichten, evtl. für die LITE Section interessant, für Leute die mit recht wenig recht viel erreichen wollen.
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that would interest me as well.
I heard from a few guys that never used more steroids and still made good gains.
I would read that, but unfortunately I don't speak 31 languages like you do ;D
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thanks for the quote, dk ::)
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Should an amateur come off totally when not compeating to save receptors or is there a low dosage for a certain period of time before trying to put on size again?
if you're planning on competing at any level, and wanting to take it further, you never come off.
test base all year long. add in compounds once or twice a year depending on your goals- bulking or cutting.
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if you're planning on competing at any level, and wanting to take it further, you never come off.
test base all year long. add in compounds once or twice a year depending on your goals- bulking or cutting.
how many years can one stay "on" before completely fucking up his nuts ?
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SHOW ME EVIDENCE OF RECEPTOR SATURATION... most get this idea because they come off and shrink a bit and then go on and make fast gains.. they figure that the "receptors" were clean and now primed for growth.. nope.. you shrunk and got back on the juice and grew... nothing more...
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I think it's beneficial to take at least a little time completely off each year, even if you are at a high level. Not because of receptors specifically but I suspect it's beneficial in various ways.
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basically impossible to be catabolic when your in a hypercaloric state .... come off gear and keep all your muscle? = come off gear and start to get fat...
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AnAl receptors no doubt
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I have actually read that testosterone increases the amount of AR:s (Androgen Receptors)..
I don't have the link to the scientific article.. it was not a "guru"-article..
I was very surprised by this facts..
Why the response to roids decreases with non-stop abuse may have other explanations..
Intake of food..
Age..
And so on..
You get the picture.
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how many years can one stay "on" before completely fucking up his nuts ?
he didn't say about fucking up his nuts. he was worried about his receptors.
:)
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basically impossible to be catabolic when your in a hypercaloric state .... come off gear and keep all your muscle? = come off gear and start to get fat...
Very true bomz.
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here is my question !!!
say you use 1g of test a week.
then you want to reduce it for a few weeks.. lets say for 10 weeks you drop it to 500mg
will you still make gain ? Or it won't do shit to your body since you used more before
"WITH A C.ERTAIN AMOUNT OF AAS YOU 4LL CARRY A CERTAIN AMOUNT OF MUSCLE,,,LOWER THE DOSAGE AND YOU LOWER THE MUSCLE YOU4LL CARRY,,,PIRIOD."""
gh15approved.
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he didn't say about fucking up his nuts. he was worried about his receptors.
:)
I am worried about that tho ;D
I want to improve, but I need my nuts or my girlfriend will fucking kick my ass when we will want to have kids in 3-4 years ! :o
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I am worried about that tho ;D
I want to improve, but I need my nuts or my girlfriend will fucking kick my ass when we will want to have kids in 3-4 years ! :o
I would do the 250iu of HCG twice weekly protocol.
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CORRECT.. there are other benefits to coming off or at the very least coming down to hrt dosage.. if on is in their 40's to come off completely is aweful in regards to maintain any mass.. the natural test levels just arent there.. and yes, genius, test does increase the number of receptor and or satelite cells... that is one theory as to why these guys keep getting bigger.. resistance training is also show to increase androgen receptors.. couple that with prolonged test use and you have the guys of today.. (along with gh ect)
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According to Dr. Anthony Goodman, in a series of lectures entitled 'Understanding the Human Body,' a cell's receptors will decrease in number when there is an excess of the corresponding hormone.
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as far as receptors goes, there is no conlcusive evidence t support receptor number increasing or decreasing from aas.
the two legit studies i can remember... they showed that at first the subjects who were on 600mg test had a big increase in AR number, but over 6 months it returned to baseline. then another one that showed a small decline in AR number.
as lats mentioned exercise boosts AR number on its own. and eating carbs should help keep ar high as well. (some kind of anti AR metabolism effect of insulin IIRC)
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Long story short (which is amazing considering I'm a meltdown machine), no one really needs to worry about androgen receptor down regulation, optimization, etc. Unless you're taking grams of androgens, using every other PED and making no progress then it is of no concern.
I think a lot of f*gs mistake AR downreg for the accumulation of SHBG elevation, being too fat and not dieting properly and using bunk or underdosed gear. That and a lot of people just become lazy after using AAS and other PEDs for too long. I've been off the sauce for longer than I wanted, but I've come to appreciate it more than anything. It was a good reality check.
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I'd like to see what a lot of you sauced-up guys look like, seriously.
No flame, but there are a lot of dudes here who hit the gear pretty good and don't post a pic
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I think a lot of f*gs mistake AR downreg for the accumulation of SHBG elevation,
well actually dont be so sure that this is a bad thing.
its possible that bound androgens can still exer their effects on AR.....and bound androgens are kept safe from liver metabolism....which means that they will be in circulation for alot longer than free androgens...
its possible shbg is beneficial
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protein and creatin receptors are utmost important to save, so you have to cycle protein and creatin.
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well actually dont be so sure that this is a bad thing.
its possible that bound androgens can still exer their effects on AR.....and bound androgens are kept safe from liver metabolism....which means that they will be in circulation for alot longer than free androgens...
its possible shbg is beneficial
Yeah I wouldn't worry about SHBG elevation, especially since all steroids reduce it. :D You don't need Proviron to combat your sky-high SHBG. :D But even if it was elevated a bit it might not be a negative like you said.
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Can the body only use a certain amount and the rest is overspill? (like pouring sugar into a spoon and it spills over after awhile) The same goes for Creatine, Protein, etc... Is there limits, without waisting sups and $$$?
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Can the body only use a certain amount and the rest is overspill? (like pouring sugar into a spoon and it spills over after awhile) The same goes for Creatine, Protein, etc... Is there limits, without waisting sups and $$$?
The sides just become much more difficult to handle.
8)
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I would read that, but unfortunately I don't speak 31 languages like you do ;D
Sorry guys, i will translate the important parts.
Training for 14 years, 10 years on roids. Because of high blood pressure (10 years on ephedrine and roids) he goes low dose and still has good results.
50mg Testo P eod
0,5ml TriTren (BD) eod
10mg Stano ed
that's 150mg testo P, 300mg TrenMix (Acetat, Hexa, Enanthate) and 70mg Stano PER WEEK.
Comments?
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GH15 is too busy sucking cocks for drug money to answer questions at this time.
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Should an amateur come off totally when not compeating to save receptors or is there a low dosage for a certain period of time before trying to put on size again?
come off for what? you wont get no where if come off,,you come off for a month sometimes when yuore poor and suffer for that 1 month ,,most smart amatuer know that moment they come off gear they go on gh and insulin or gh so never off
BODYBUILDER NEVER OFF NEVER OFF NEVER OFF WE NEVER OFF EVERYTHING,,ALWAYS ON SOMETHING,,YOU WILL NEVER FIND A BODYBUILDER COMPLETLY OFF EVERYTHING,,NOT EXISTED AS OF 2009 UNLESS SICK OR NOT COMPETING AND DONT CARE ANYMORE BUT EVEN WHEN NOT COMPETING THEY ALL HAVE THE MENTAL THING,,ITS A DEASEASE ,,ONCE YOURE IN YOU VERY RARELY GO OUT UNLESS YOUR LIFE IS AT RISK AND MOST WOULD NOT GIVE A DAMN AND RISK THEIR LIFE UNLESS SOMETHING FAILS LIKE KIDNY ETC AND EVEN THEN YOU SEE THE GENIOUSES WHO GET BACK ON THE STUFF RIGHT AFTER DYALASYS ,,
BODYBUILDERS = ADDICTS
LEARN IT
MJ15 APPROVED
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someone please save my receptor
LOL ;D
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come off for what? you wont get no where if come off,,you come off for a month sometimes when yuore poor and suffer for that 1 month ,,most smart amatuer know that moment they come off gear they go on gh and insulin or gh so never off
BODYBUILDER NEVER OFF NEVER OFF NEVER OFF WE NEVER OFF EVERYTHING,,ALWAYS ON SOMETHING,,YOU WILL NEVER FIND A BODYBUILDER COMPLETLY OFF EVERYTHING,,NOT EXISTED AS OF 2009 UNLESS SICK OR NOT COMPETING AND DONT CARE ANYMORE BUT EVEN WHEN NOT COMPETING THEY ALL HAVE THE MENTAL THING,,ITS A DEASEASE ,,ONCE YOURE IN YOU VERY RARELY GO OUT UNLESS YOUR LIFE IS AT RISK AND MOST WOULD NOT GIVE A DAMN AND RISK THEIR LIFE UNLESS SOMETHING FAILS LIKE KIDNY ETC AND EVEN THEN YOU SEE THE GENIOUSES WHO GET BACK ON THE STUFF RIGHT AFTER DYALASYS ,,
BODYBUILDERS = ADDICTS
LEARN IT
MJ15 APPROVED
what do you say about this cycle?
50mg Testo P eod
0,5ml TriTren (BD) eod
10mg Stano ed
that's 150mg testo P, 300mg TrenMix (Acetat, Hexa, Enanthate) and 70mg Stano PER WEEK.
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UNLESS SOMETHING FAILS LIKE KIDNY ETC AND EVEN THEN YOU SEE THE GENIOUSES WHO GET BACK ON THE STUFF RIGHT AFTER DYALASYS ,,
;D
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come off for what? you wont get no where if come off,,you come off for a month sometimes when yuore poor and suffer for that 1 month ,,most smart amatuer know that moment they come off gear they go on gh and insulin or gh so never off
BODYBUILDER NEVER OFF NEVER OFF NEVER OFF WE NEVER OFF EVERYTHING,,ALWAYS ON SOMETHING,,YOU WILL NEVER FIND A BODYBUILDER COMPLETLY OFF EVERYTHING,,NOT EXISTED AS OF 2009 UNLESS SICK OR NOT COMPETING AND DONT CARE ANYMORE BUT EVEN WHEN NOT COMPETING THEY ALL HAVE THE MENTAL THING,,ITS A DEASEASE ,,ONCE YOURE IN YOU VERY RARELY GO OUT UNLESS YOUR LIFE IS AT RISK AND MOST WOULD NOT GIVE A DAMN AND RISK THEIR LIFE UNLESS SOMETHING FAILS LIKE KIDNY ETC AND EVEN THEN YOU SEE THE GENIOUSES WHO GET BACK ON THE STUFF RIGHT AFTER DYALASYS ,,
BODYBUILDERS = ADDICTS
LEARN IT
MJ15 APPROVED
"""HI FERLLAS,,,IN THE LIFE WHEN YOU WANT SOME.THING YOU MUST DO SCACRIFISE,,,COME OFF AND YOU 4LL STAY A LOCAL AMATEUR SO IF YOU WANT TO SAVE RECEPTOR JUST SWITCH WITH OTHER ROID.Z JUST SWITCH FROM TEST TO TREN OR GRAPEFRUIT"""
GH15 APPROVED
i was right.
i'm into the mind of gh15.
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well actually dont be so sure that this is a bad thing.
its possible that bound androgens can still exer their effects on AR.....and bound androgens are kept safe from liver metabolism....which means that they will be in circulation for alot longer than free androgens...
its possible shbg is beneficial
True enough. But that, myostatin, prostaglandins, cytokines, all these millions of eccentric growth factors that we know nothing about, even progestin and prolactin, no one has really looked at the grand scheme of things in a bodybuilding application. There are plenty of endocrinologists and studies out there, but nothing focused towards a supraphysiological level of androgens being taken for the purpose of building muscle.
All we have are studies showing the androgenic and anabolic affects of sex steroids applied to rat muscle, and the hepatotoxic affects of large doses of androgens. We've got a few human studies on people with third degree burns and inherent wasting syndrome from chronic and irreparable illness. I don't think that we'll ever know, but I don't think we should immediately chalk it up to AR down regulation.
I remember, yeaarrs ago, I think it was Bill Lewellyn and Par Deus or some other AAS guru out there duking it out over AR down regulation. I think Patrick Arnold and Bruce Kneller joined in some heated debates too. Those were gold. But when I read them it was years before I even picked up a weight and I was barely over 100lbs, didn't even know what protein was but was interested in the science of getting big biceps. Anyone have a link to any of these old threads?
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the reason you still look good at the lower doses when u drop down is that your keeping your self in an anabolic state, after so long gear acts as more as an anti catabolic.....
mark my words, if u want to go places dont come off,, blast and cruise.......
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what do you say about this cycle?
50mg Testo P eod
0,5ml TriTren (BD) eod
10mg Stano ed
that's 150mg testo P, 300mg TrenMix (Acetat, Hexa, Enanthate) and 70mg Stano PER WEEK.
crap. dont shoot prop or acetate eod.
winny is a waste at 10mg.
wouldnt use BD tri tren ever... no fucking reason at all for mixed esters..overpriced garbage. the hexa ester is basically the same as the enanthate ester...anyone stupid enough to produce a steroid with acetate, enanthate, hex is nto smart enough to make a steroid iw ould buy. at least have some base knowledge befor eyou start making oils that will be injected into peoples bodies.
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True enough. But that, myostatin, prostaglandins, cytokines, all these millions of eccentric growth factors that we know nothing about, even progestin and prolactin, no one has really looked at the grand scheme of things in a bodybuilding application.
absolutely.
which is why anecdotal evidence reigns supreme, as of now.
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absolutely.
which is why anecdotal evidence reigns supreme, as of now.
Yeah, I'm not sure why most people here sweat it though. Aside from the few pros that are still on board, no one should be having problems with their androgen receptors. Just up the dose, use stronger anabolics and make sure that what you're injecting is REAL and not some bathtub brew. 8)
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The reasons that people often stop having gains at week 8-9 has nothing to do with androgen receptors.
It is more than likely linked to the fact that supra-physiological doses of testosterone up-regulate myostatin, inhibiting growth.
This does seem to taper off again at week twenty, so you can avoid this with short cycles or longer cycles.
Another endocrine interaction is that testosterone induces insulin resistance in muscle, so the anabolic effects of insulin are muted.
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Can the body only use a certain amount and the rest is overspill? (like pouring sugar into a spoon and it spills over after awhile) The same goes for Creatine, Protein, etc... Is there limits, without waisting sups and $$$?
Didn't get to much feedback on this part. Anybody else have any comments on this?
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The reasons that people often stop having gains at week 8-9 has nothing to do with androgen receptors.
It is more than likely linked to the fact that supra-physiological doses of testosterone up-regulate myostatin, inhibiting growth.
This does seem to taper off again at week twenty, so you can avoid this with short cycles or longer cycles.
Another endocrine interaction is that testosterone induces insulin resistance in muscle, so the anabolic effects of insulin are muted.
where do you come up with this stuff, woman?
AFAIK test slighty decreases myostatin...
as far s insulin resistance, yes, however bodybuilders dont really get this as they are constantly depleting glycogen stopres through training which re-sensitizes them to insulin.
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crap. dont shoot prop or acetate eod.
winny is a waste at 10mg.
wouldnt use BD tri tren ever... no fucking reason at all for mixed esters..overpriced garbage. the hexa ester is basically the same as the enanthate ester...anyone stupid enough to produce a steroid with acetate, enanthate, hex is nto smart enough to make a steroid iw ould buy. at least have some base knowledge befor eyou start making oils that will be injected into peoples bodies.
Well, maybe you should try it.
If a guy that megadosed for ten years likes it, it cannot be that bad.
I don't want to miscredit you, but the poster was the gh15 of a famous german board, he ran a famous german underground lab (maybe the most hyped UG lab in Germany ever) and looked quite decent.
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Well, maybe you should try it.
If a guy that megadosed for ten years likes it, it cannot be that bad.
I don't want to miscredit you, but the poster was the gh15 of a famous german board, he ran a famous german underground lab (maybe the most hyped UG lab in Germany ever) and looked quite decent.
i didnt say it w2ouldnt work i just said it was crap as far as possibilities go.
of course itll work, its juice! :)
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where do you come up with this stuff, woman?
AFAIK test slighty decreases myostatin...
as far s insulin resistance, yes, however bodybuilders dont really get this as they are constantly depleting glycogen stopres through training which re-sensitizes them to insulin.
What is AFAIK testosterone?
This is where I got it from.
: Mol Cell Endocrinol. 2009 Apr 10;302(1):26-32. Epub 2009 Jan 21. Links
Measurement of myostatin concentrations in human serum: Circulating concentrations in young and older men and effects of testosterone administration.
Lakshman KM, Bhasin S, Corcoran C, Collins-Racie LA, Tchistiakova L, Forlow SB, St Ledger K, Burczynski ME, Dorner AJ, Lavallie ER.
Section of Endocrinology, Diabetes, and Nutrition, Boston University School of Medicine, Boston Medical Center, 670 Albany Street, Boston, MA 02118, United States.
Methodological problems, including binding of myostatin to plasma proteins and cross-reactivity of assay reagents with other proteins, have confounded myostatin measurements. Here we describe development of an accurate assay for measuring myostatin concentrations in humans. Monoclonal antibodies that bind to distinct regions of myostatin served as capture and detector antibodies in a sandwich ELISA that used acid treatment to dissociate myostatin from binding proteins. Serum from myostatin-deficient Belgian Blue cattle was used as matrix and recombinant human myostatin as standard. The quantitative range was 0.15-37.50 ng/mL. Intra- and inter-assay CVs in low, mid, and high range were 4.1%, 4.7%, and 7.2%, and 3.9%, 1.6%, and 5.2%, respectively. Myostatin protein was undetectable in sera of Belgian Blue cattle and myostatin knockout mice. Recovery in spiked sera approximated 100%. ActRIIB-Fc or anti-myostatin antibody MYO-029 had no effect on myostatin measurements when assayed at pH 2.5. Myostatin levels were higher in young than older men (mean+/-S.E.M. 8.0+/-0.3 ng/mL vs. 7.0+/-0.4 ng/mL, P=0.03). In men treated with graded doses of testosterone, myostatin levels were significantly higher on day 56 than baseline in both young and older men; changes in myostatin levels were significantly correlated with changes in total and free testosterone in young men. Myostatin levels were not significantly associated with lean body mass in either young or older men.
CONCLUSION: Myostatin ELISA has the characteristics of a valid assay: nearly 100% recovery, excellent precision, accuracy, and sufficient sensitivity to enable measurement of myostatin concentrations in men and women.
PMID: 19356623 [PubMed - in process]
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What is AFAIK testosterone?
This is where I got it from.
: Mol Cell Endocrinol. 2009 Apr 10;302(1):26-32. Epub 2009 Jan 21. Links
Measurement of myostatin concentrations in human serum: Circulating concentrations in young and older men and effects of testosterone administration.
Lakshman KM, Bhasin S, Corcoran C, Collins-Racie LA, Tchistiakova L, Forlow SB, St Ledger K, Burczynski ME, Dorner AJ, Lavallie ER.
Section of Endocrinology, Diabetes, and Nutrition, Boston University School of Medicine, Boston Medical Center, 670 Albany Street, Boston, MA 02118, United States.
Methodological problems, including binding of myostatin to plasma proteins and cross-reactivity of assay reagents with other proteins, have confounded myostatin measurements. Here we describe development of an accurate assay for measuring myostatin concentrations in humans. Monoclonal antibodies that bind to distinct regions of myostatin served as capture and detector antibodies in a sandwich ELISA that used acid treatment to dissociate myostatin from binding proteins. Serum from myostatin-deficient Belgian Blue cattle was used as matrix and recombinant human myostatin as standard. The quantitative range was 0.15-37.50 ng/mL. Intra- and inter-assay CVs in low, mid, and high range were 4.1%, 4.7%, and 7.2%, and 3.9%, 1.6%, and 5.2%, respectively. Myostatin protein was undetectable in sera of Belgian Blue cattle and myostatin knockout mice. Recovery in spiked sera approximated 100%. ActRIIB-Fc or anti-myostatin antibody MYO-029 had no effect on myostatin measurements when assayed at pH 2.5. Myostatin levels were higher in young than older men (mean+/-S.E.M. 8.0+/-0.3 ng/mL vs. 7.0+/-0.4 ng/mL, P=0.03). In men treated with graded doses of testosterone, myostatin levels were significantly higher on day 56 than baseline in both young and older men; changes in myostatin levels were significantly correlated with changes in total and free testosterone in young men. Myostatin levels were not significantly associated with lean body mass in either young or older men.
CONCLUSION: Myostatin ELISA has the characteristics of a valid assay: nearly 100% recovery, excellent precision, accuracy, and sufficient sensitivity to enable measurement of myostatin concentrations in men and women.
PMID: 19356623 [PubMed - in process]
Stands for "as far as I know".
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Stands for "as far as I know".
LOL, ta.
I thought it was some molecular biology term for one of the proteins, genes or receptors like mTor, bax, p53.
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LOL, ta.
I thought it was some molecular biology term for one of the proteins, genes or receptors like mTor, bax, p53.
Do you know what YEM means. ;)
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Do you know what YEM means. ;)
Yeast extracted mannitol.
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Yeast extracted mannitol.
lol no..
You excite me ;) :-*
Your number 1 online stalker ;D
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What is AFAIK testosterone?
This is where I got it from.
: Mol Cell Endocrinol. 2009 Apr 10;302(1):26-32. Epub 2009 Jan 21. Links
Measurement of myostatin concentrations in human serum: Circulating concentrations in young and older men and effects of testosterone administration.
Lakshman KM, Bhasin S, Corcoran C, Collins-Racie LA, Tchistiakova L, Forlow SB, St Ledger K, Burczynski ME, Dorner AJ, Lavallie ER.
Section of Endocrinology, Diabetes, and Nutrition, Boston University School of Medicine, Boston Medical Center, 670 Albany Street, Boston, MA 02118, United States.
Methodological problems, including binding of myostatin to plasma proteins and cross-reactivity of assay reagents with other proteins, have confounded myostatin measurements. Here we describe development of an accurate assay for measuring myostatin concentrations in humans. Monoclonal antibodies that bind to distinct regions of myostatin served as capture and detector antibodies in a sandwich ELISA that used acid treatment to dissociate myostatin from binding proteins. Serum from myostatin-deficient Belgian Blue cattle was used as matrix and recombinant human myostatin as standard. The quantitative range was 0.15-37.50 ng/mL. Intra- and inter-assay CVs in low, mid, and high range were 4.1%, 4.7%, and 7.2%, and 3.9%, 1.6%, and 5.2%, respectively. Myostatin protein was undetectable in sera of Belgian Blue cattle and myostatin knockout mice. Recovery in spiked sera approximated 100%. ActRIIB-Fc or anti-myostatin antibody MYO-029 had no effect on myostatin measurements when assayed at pH 2.5. Myostatin levels were higher in young than older men (mean+/-S.E.M. 8.0+/-0.3 ng/mL vs. 7.0+/-0.4 ng/mL, P=0.03). In men treated with graded doses of testosterone, myostatin levels were significantly higher on day 56 than baseline in both young and older men; changes in myostatin levels were significantly correlated with changes in total and free testosterone in young men. Myostatin levels were not significantly associated with lean body mass in either young or older men.
CONCLUSION: Myostatin ELISA has the characteristics of a valid assay: nearly 100% recovery, excellent precision, accuracy, and sufficient sensitivity to enable measurement of myostatin concentrations in men and women.
PMID: 19356623 [PubMed - in process]
ahahhaaa, True Adonis on a copy paste spread again.