Looks like some ineteresting research, I assume you did alot of immunofluresence work, with specific Ab's to the Raf1 and PKC and developed nice photos of these two proteins localized together on some cytoskeletal proteins in these THP-1 cells. I guess the next two steps would be to develop a way to inhibit their interaction by. finding the ammino acid residue where binding is occuring (using recombinant proteins - this could be very hard) or accually using the Ab's to try and interfere with their binding. But this could be a serious task if your protein has 300 binding partners. That TA is a true dickhead, your work is beyond the title of exersize science - your shyt is straight cell\molecular biology. I'm currently working on a PhD purifying and characterizing a cell-wall protein PGIP (polygalacturonase-inhibiting protein) from strawberry plants and trying to extract proteins from the cell-wall of plants is no joke!!
First off, let me say thank you to not just you, but everyone on the board that have had such supportive things to say regarding this. I didn't think anyone on here would care.
Secondly, Vince, the day I am at "your level" is the day I will shoot myself. That may come off as personal, and maybe it is, however, you have made an extarordinary ass of yourself on here. Adonis isn't even worth responding to on these boards. His comment was neither witty nor pertinent.
Anyways, there was a tonne of immunofluorescence work done during the completion of my thesis. You are absolutely correct that the use of interference peptides may be of some value in elucidating the exact mechanisms of the interactions I observed during the course of my experiments. There are a huge number of additional experiments that have to be done in order to complete this tiny picture in the huge picture of cell signaling. Furthermore, you are absolutely correct that inhibiting the function of 14-3-3 proteins may be difficult for a number of reasons, however, there is some good news: their number in the cell may be finite insofar as availability is concerned and may be a mechanism through which cell signaling is regulated via these proteins.
To those of you doing a PhD, I applaud you. That is a long arduous road and I have nothing but respect for you regarding that. By the way I am really sorry I am not mentioning everyone individually, but, after having just finished, I am feeling kind of tired of typing.
Also, in regards to the pertinence of this work to hypertrophy, there is most definetly some cross over. I understand that my project was very specific, however, the component parts of cell signaling that I studied are ubiquitously expressed in cells and play roles in growth, differentiation and apoptosis, all of which are most definetly important processes regarding muscular growth. I can not tell you how, however, these enzymes are of critical importance in tissue growth and development and thus, if understood at even a basic level, it would make reading more specific research papers much, much easier.
Anyways, if anyone wants links to rather broad reviews of the Raf-1 kinase, the PKC kinase family or 14-3-3 proteins, I would be happy to oblige. Thanks again Vince (B) and everyone else for their well wishes. To the others, keep up the good work, you make this place what it is
