Getbig.com: American Bodybuilding, Fitness and Figure
Getbig Bodybuilding Boards => Steroids Info & Hardcore => Topic started by: Fatpanda on January 01, 2009, 04:03:20 PM
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anyone know the patent number on the manufacture/preperation on oxymetholone ?
i can't seem to find it anywhere, and its driving me nuts.
also any chemists/ medicinal chemists here ? i have a question or 2 about a pharmacopoeia.
cheers.
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get yourself a PDR.
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get yourself a PDR.
??? pdr?
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??? pdr?
Physicians Desk Reference, it's a huge 5 inch thick book doctor's use to find drug information.
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Physicians Desk Reference, it's a huge 5 inch thick book doctor's use to find drug information.
that doesn't have the info i require.
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that doesn't have the info i require.
how do you know, have you ever seen a PDR? that thing has VERY detailed info on almost every drug that is made by any company INCLUDING Anadrol aka Oxymetholone, molecular structures, half lifes, contraindications, side effects, etc., google it.
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how do you know, have you ever seen a PDR? that thing has VERY detailed info on almost every drug that is made by any company INCLUDING Anadrol aka Oxymetholone, molecular structures, half lifes, contraindications, side effects, etc., google it.
yes - it has no patents, no details on chemical processes to manufacture it, it is a basic reference guide for a doctor in the things you mentioned - detailed, but basic.
i have the details i require for the manufactiure of every single drug a bodybuilder would ever require ( and more) except oxymetholone. i think the patent must be protected, but i would like to know when it expires if anyone knows.
thanks for your help though quaker.
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yes - it has no patents, no details on chemical processes to manufacture it, it is a basic reference guide for a doctor in the things you mentioned - detailed, but basic.
i have the details i require for the manufactiure of every single drug a bodybuilder would ever require ( and more) except oxymetholone. i think the patent must be protected, but i would like to know when it expires if anyone knows.
thanks for your help though quaker.
contact Unimed and see what they say. ;D
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i think thats because oxymetholone doesnt have a patent. well, thats because i know of a few different pharmacies that make it.
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i think thats because oxymetholone doesnt have a patent. well, thats because i know of a few different pharmacies that make it.
the original Syntex stuff was unreal.
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the original Syntex stuff was unreal.
never tried it. i tried legit human grade drol from dritish dispencary... and that stuff made me feel horrible.. like i had a mild comedown off coke or something... for me i dont like anadrol at all
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contact Unimed and see what they say. ;D
i don't think unimed own the patent. i know they own the rights in america to sell anadrol, but i think syntex still hold the patent. unless unimed bought the entire patent from them.
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i think thats because oxymetholone doesnt have a patent. well, thats because i know of a few different pharmacies that make it.
i don't think so, as i can't find details of it. if its generic do you know where can i find its details ?
i may already have it in that pharmacopoeia i mentioned, but i am still unclear. :-\ it seems like it explains its manufacture, but i currently lack the chemical/medicinal knowlege needed to make sense of it.
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never tried it. i tried legit human grade drol from dritish dispencary... and that stuff made me feel horrible.. like i had a mild comedown off coke or something... for me i dont like anadrol at all
give it another shot only this time cut it in half and take it with food, i know Palumbo doesn't believe in it because he thinks it interferes with the appetite.
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give it another shot only this time cut it in half and take it with food, i know Palumbo doesn't believe in it because he thinks it interferes with the appetite.
have you tried it quaker ?
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have you tried it quaker ?
don't know what you're talking about. :o
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don't know what you're talking about. :o
the cock of course.
hypothetically speaking, do you feel if you were a bodybuilder or powerlifter looking for extreme gains adrol would be benificial?
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the cock of course.
hypothetically speaking, do you feel if you were a bodybuilder or powerlifter looking for extreme gains adrol would be benificial?
hahahhaa, hell yes it would be beneficial!!, only thing stronger is maybe test suspension, probably EVERY great powerlifter or bodybuilder has used it at one point or another, gauranteed blood volume increase of 15% in the body after taking it for a few weeks.
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the studies i have read back this up 100%. i will have to try it soon.
as i said i would really like to find the patent/details of manufacture though.
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give it another shot only this time cut it in half and take it with food, i know Palumbo doesn't believe in it because he thinks it interferes with the appetite.
okay i will try it...
i think palumbo is used to orals for a loooonnnnngggg time or at a hhhiiiiigggghhhh doseage (or both)..because thats the only time i think orals will fuck with appetite.
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okay i will try it...
i think palumbo is used to orals for a loooonnnnngggg time or at a hhhiiiiigggghhhh doseage (or both)..because thats the only time i think orals will fuck with appetite.
it is a side effect of its use - says so on the packet.
also i have read this effect happens to lots of users who take adrol - i haven't used it myself though.
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it is a side effect of its use - says so on the packet.
also i have read this effect happens to lots of users who take adrol - i haven't used it myself though.
if a person takes it with food they should be fine, just don't take a couple with your morning coffee on an empty stomach.
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if a person takes it with food they should be fine, just don't take a couple with your morning coffee on an empty stomach.
theoretically how high a dosage each day have you taken - if this discussion were not complete fantasy ?
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theoretically how high a dosage each day have you taken - if this discussion were not complete fantasy ?
i think a person would do awesome with between 50-100mg. a day, pumps would be insane.
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i think a person would do awesome with between 50-100mg. a day, pumps would be insane.
theoretically has fictional character close to you ever went above 100mg a day ?
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theoretically has fictional character close to you ever went above 100mg a day ?
no reason to.
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no reason to.
science agrees. just wondered, as we have all read of the adrol rambos who swear by 200mg + a day. ::)
good stuff quaker.
whats this fictional character's numbers in the big 3 these days ?
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science agrees. just wondered, as we have all read of the adrol rambos who swear by 200mg + a day. ::)
good stuff quaker.
whats this fictional character's numbers in the big 3 these days ?
oh that's not to say there haven't been peoplwho take way more than that, i heard rumors of Cutler supposedly taking 7 a day, like i said they're rumors but you know how pros are, if a little is good a lot must be great.
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science agrees. just wondered, as we have all read of the adrol rambos who swear by 200mg + a day. ::)
good stuff quaker.
whats this fictional character's numbers in the big 3 these days ?
i would disgaree- science does not agree.
with androgens, more is better, at least for "bodybuilding" - not health.
i know of people, not even pros or amatuers or competitors, who have taken as many as 6 per day (300mg ). kid i knew in high school on the football team. squatted 5 plates and benched 4.
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i would disgaree- science does not agree.
with androgens, more is better, at least for "bodybuilding" - not health.
i know of people, not even pros or amatuers or competitors, who have taken as many as 6 per day (300mg ). kid i knew in high school on the football team. squatted 5 plates and benched 4.
;D
it does agree in the case of anadrol - this is the one exception to that rule. i have studies to back this candy.
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;D
it does agree in the case of anadrol - this is the one exception to that rule. i have studies to back this candy.
post em up
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i think tbombz is just saying what i was saying in that these are pro bb'ers we're talking about, they don't do anything moderately.
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post em up
Double-blind, randomized, placebo-controlled phase III trial of oxymetholone for the treatment of HIV wasting.
ABSTRACT
Background: Despite highly active antiretroviral therapy (HAART), chronic involuntary weight loss of lean body mass (LBM) and body cell mass (BCM). still remains a serious problem in the care of HIV patients. Previous studies have been performed with androgen replacement therapy or treatment with recombinant growth hormone(rGH) showing partial restoration of LBM, but these treatments have largely not been assessed in eugonadal individuals.Study Design: Double-blind, randomized, placebo-controlled trial of 89 HIV-positive women and men with wasting assigned to the anabolic steroid oxymetholone (50 mg BID or TID) or placebo for 16 weeks followed by open-label treatment.Results: Oxymetholone led to a significant weight gain of 3.0 ±0.5 and 3.5 ±0.7 kg in the TID and BID groups, respectively (p<0.05 for each treatment versus placebo), while individuals in the placebo group gained an average of 1.0 ±0.7 kg. Body cell mass increased in the oxymetholone BID group (3.8 ±0.4 kg; p<0.0001) and in the oxymetholone TID group (2.1 ±0.6 kg; p<0.005), corresponding to 12.4% and 7.4% of baseline BCM, respectively. The most important adverse event was liver-associated toxicity. Overall, 35% of patients in the TID, 27% of patients in the BID oxymetholone group and no patients the placebo group had a greater than 5 times baseline increase for ALT during the double-blind phase of the study.
Conclusions: Oxymetholone can be considered an effective anabolic steroidin eugonadal male and female patients with AIDS-associated wasting. The BID (100 mg/day) regimen appeared to be equally effective to the TID (150 mg/day) regimenin terms of weight gain, LBM and BCM and was associated with less, but still significant liver toxicity.
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i think tbombz is just saying what i was saying in that these are pro bb'ers we're talking about, they don't do anything moderately.
i agree, we all know guys who go crazy with little regard for health or even results.
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well thats interestign. but that studies really only says that in people with anemia who arent training ad have lower than normal muscle mass, 150 doesnt produce any noticeabl;e more gains than 100mg.
as far as trained bodybuilders with excess muscle already, maybe even in caloric defecit, im sure the higher dose does yeiled better results.
thats just the way these things work.
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i have another study that compares 50mg and 100mg.
the 100mg had double the strength increases, but only slightly better mass increases.
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well thats interestign. but that studies really only says that in people with anemia who arent training ad have lower than normal muscle mass, 150 doesnt produce any noticeabl;e more gains than 100mg.
as far as trained bodybuilders with excess muscle already, maybe even in caloric defecit, im sure the higher dose does yeiled better results.
thats just the way these things work.
you know i agree with you on this for most drugs, but not on this.
all drugs have a tipping point, this study shows me that the tipping point for adrol is 100mg.
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i just dont agree with you. andorgens may have a poitn at which the increases in benefits do not equal the price they are worth or the side effects they incur, however, simply speaking about increasing protien synthesis, response to training, lowering cortisol, etc etc etc... more will always be better with these drugs.
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i just dont agree with you. andorgens may have a poitn at which the increases in benefits do not equal the price they are worth or the side effects they incur, however, simply speaking about increasing protien synthesis, response to training, lowering cortisol, etc etc etc... more will always be better with these drugs.
only up to a point.
standard AR theory states there is a limit to how much steroid a body can use, due to a limited amount of receptors ( i am aware anadrol does not bind strongly to the AR, before you say it) but whatever mechanism it works via it will attach to some sort of receptor of which there will be a finite amount.
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for patent check http://www.uspto.gov/index.html
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for patent check http://www.uspto.gov/index.html
already did during my search benz, its not there :(
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already did during my search benz, its not there :(
prolly expired, in pharma stuff nothing last forever
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only up to a point.
standard AR theory states there is a limit to how much steroid a body can use, due to a limited amount of receptors ( i am aware anadrol does not bind strongly to the AR, before you say it) but whatever mechanism it works via it will attach to some sort of receptor of which there will be a finite amount.
all androgens work via the ar no nmatter the binding affinity
binding affinity is pretty much useless
for example
binding affinity refers to the "amount of binding" that takes place with any given androgen. but, what mattes is not the quantity of the bind, but the quality. for exmaple, a androgen may bind tightly, and activate multiple genes, but if those arent good things for muscle growth, then thats a weak androgen. now, another androgen migt bind very loosely and only activate one ting, but if thats somethign that very strongly activates protein synthesis, then thats a STRONG androgen.
as for the ar theory, thats invalid, because ANDROGENS ARE INVOLVED WITH THE PRODUCTION OF AR. which means, the more androgens you put in more body, the more androgen receptors your body manufactores.
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prolly expired, in pharma stuff nothing last forever
Five years of monopoly/patent
Then its free market
Its to promote research and development
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as for the ar theory, thats invalid, because ANDROGENS ARE INVOLVED WITH THE PRODUCTION OF AR. which means, the more androgens you put in more body, the more androgen receptors your body manufactores.
Thats all good and well but;
How come results with dbol for example seem to taper off after 40-60mgs ED?
Van Bilderass would agree with that
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Thats all good and well but;
How come results with dbol for example seem to taper off after 40-60mgs ED?
Van Bilderass would agree that
i can only tell you that if results taper offf for you at 4-60mgs, then it may b because the steroid is getting converted by aromatase or alpha-2 reductase
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i can only tell you that if results taper offf for you at 4-60mgs, then it may b because the steroid is getting converted by aromatase or alpha-2 reductase
exactly... then how would extra androgens be stimulating AR production?
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exactly... then how would extra androgens be stimulating AR production?
good point. probabaly need adex finasteride or soething like that
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Five years of monopoly/patent
Then its free market
Its to promote research and development
any ideas on oxymetholone then ?
all androgens work via the ar no nmatter the binding affinity
binding affinity is pretty much useless
for example
binding affinity refers to the "amount of binding" that takes place with any given androgen. but, what mattes is not the quantity of the bind, but the quality. for exmaple, a androgen may bind tightly, and activate multiple genes, but if those arent good things for muscle growth, then thats a weak androgen. now, another androgen migt bind very loosely and only activate one ting, but if thats somethign that very strongly activates protein synthesis, then thats a STRONG androgen.
as for the ar theory, thats invalid, because ANDROGENS ARE INVOLVED WITH THE PRODUCTION OF AR. which means, the more androgens you put in more body, the more androgen receptors your body manufactores.
that does not make the AR theory invalid ::)
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any ideas on oxymetholone then ?
that does not make the AR theory invalid ::)
dude... oxymetholone was released in 1960...
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dude... oxymetholone was released in 1960...
i know, so were others but i have found them. :D
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any ideas on oxymetholone then ?
that does not make the AR theory invalid ::)
well, yes it does, people think that after a certain dose, that all ar will be in use, however, its doesnt work like that. androgens bind for a very short while then they move on and bind t another, then they leave and bind to another. etc etc etc. untill they are metabolized by the body. the more ar the body has, the more work an androgen can do before it is metabolized. with increasing amounts of anddrogens, the body makes more ar.
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well, yes it does, people think that after a certain dose, that all ar will be in use, however, its doesnt work like that. androgens bind for a very short while then they move on and bind t another, then they leave and bind to another. etc etc etc. untill they are metabolized by the body. the more ar the body has, the more work an androgen can do before it is metabolized. with increasing amounts of anddrogens, the body makes more ar.
candy thats not what happens. if the compound doesn't bind to the AR it gets excreted via urine or feces.
thats why chemists invented esters, to avoids this waste.
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candy thats not what happens. if the compound doesn't bind to the AR it gets excreted via urine or feces.
thats why chemists invented esters, to avoids this waste.
hes an expert, watch your mouth convict ::)
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candy thats not what happens. if the compound doesn't bind to the AR it gets excreted via urine or feces.
thats why chemists invented esters, to avoids this waste.
its excactly what happens.
esters prolong the ability for a steroid to become active. steroid isnt active untill the ester is cleaved. then it starts to go to work. it moves trough the body..binding to ar then leaving then binding to another ar untill its metabolized (this means it destroyed by the body...or also like you said it can be excreted too).
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its excactly what happens.
esters prolong the ability for a steroid to become active. steroid isnt active untill the ester is cleaved. then it starts to go to work. it moves trough the body..binding to ar then leaving then binding to another ar untill its metabolized (this means it destroyed by the body...or also like you said it can be excreted too).
Watch your mouth convict
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its excactly what happens.
esters prolong the ability for a steroid to become active. steroid isnt active untill the ester is cleaved. then it starts to go to work. it moves trough the body..binding to ar then leaving then binding to another ar untill its metabolized (this means it destroyed by the body...or also like you said it can be excreted too).
they don't bind to 1 then move off and bind to another, they lock, get used and are done.
the ones that don't bind get excreted. they don't hang about.
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they don't bind to 1 then move off and bind to another, they lock, get used and are done.
the ones that don't bind get excreted. they don't hang about.
no thats incorrect. how i explained it to you is accurate.
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no thats incorrect. how i explained it to you is accurate.
watch your mouth convict
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Fatpanda you say you have the manufactoring process in the book? Post it up and I'll decipher it for you
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Fatpanda you say you have the manufactoring process in the book? Post it up and I'll decipher it for you
how
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I'm studying medicinal chemistry ;)
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I'm studying medicinal chemistry ;)
beautiful, can you elaborate bombs?
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Fatpanda you say you have the manufactoring process in the book? Post it up and I'll decipher it for you
cheers, give me a few mins.
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Procedure— Proceed as directed for Procedure under 'Single-steroid Assay 511' (see below), using a solvent system consisting of a mixture of benzene and alcohol (98:2), through the fourth sentence of the second paragraph under Procedure. Then centrifuge the tubes for 5 minutes, and determine the absorbances of the supernatants in 1-cm cells at the wavelength of maximum absorbance at about 315 nm, with a suitable spectrophotometer, against the blank. [NOTE—Use 0.01 N alcoholic sodium hydroxide, rather than alcohol, to elute the silica gel bands.] Calculate the quantity, in mg, of C21H32O3 in the portion of Oxymetholone taken by the formula:
10C(AU / AS), in which C is the concentration, in mg per mL, of USP Oxymetholone RS (see below) in the Standard preparation, and AU and AS are the absorbances of the solutions from the Assay preparation and the Standard preparation, respectively.
single-steroid assay 511 - In the following procedure, the steroid to be assayed is separated from related foreign steroids and excipients by thin-layer chromatography and determined following recovery from the chromatogram.
Preparation of the Plate— Prepare a slurry from 30 g of chromatographic silica gel with a suitable fluorescing substance by the gradual addition, with mixing, of about 65 mL of a mixture of water and alcohol (5:2). Transfer the slurry to a clean, 20- × 20-cm plate, spread to make a uniform layer 250 µm thick, and allow to dry at room temperature for 15 minutes. Heat the plate at 105 for 1 hour, and store in a desiccator.
Solvent A— Mix methylene chloride with methanol (180:16).
Solvent B— Mix chloroform with acetone (4:1).
Standard Preparation— Dissolve in a mixture of equal volumes of chloroform and alcohol a suitable quantity of the USP Reference Standard specified in the individual monograph, previously dried as directed (see USP Reference Standards 11) and accurately weighed, to obtain a solution having a known concentration of about 2 mg per mL.
Assay Preparation— Prepare as directed in the individual monograph.
Procedure— Divide the area of the chromatographic plate into three equal sections, the left and right sections to be used for the Assay Preparation and the Standard Preparation, respectively, and the center section for the blank. Apply 200 µL each of the Assay Preparation and the Standard Preparation as streaks 2.5 cm from the bottom of the appropriate section of the plate. Dry the solution as it is being applied, with the aid of a stream of air. Using the Solvent specified in the individual monograph, develop the chromatogram in a suitable chamber, previously equilibrated and lined with absorbent paper, until the solvent front has moved 15 cm above the initial streaks.
Remove the plate, evaporate the solvent, and locate the principal band occupied by the Standard Preparation by viewing under UV light. Mark this band, as well as corresponding bands in the Assay Preparation and blank sections of the plate. Remove the silica gel from each band separately, either by scraping onto glazed weighing papers or by using a suitable vacuum collecting device, and transfer it to a glass-stoppered, 50-mL centrifuge tube. To each tube add 25.0 mL of alcohol, and shake for not less than 2 minutes. Centrifuge the tubes for 5 minutes, pipet 20 mL of the supernatant from each tube into a glass-stoppered, 50-mL conical flask, add 2.0 mL of a solution prepared by dissolving 50 mg of blue tetrazolium in 10 mL of methanol, and mix. Proceed as directed for Procedure under Assay for Steroids 351, beginning with “Then to each flask.”
USP Oxymetholone RS— Dry portion in vacuum over phosphorus pentoxide for 4 hours before using. Keep container tightly closed.
is this what i'm after ?
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There's really not much to decipher there, you would need a well equiped lab to do some of that stuff, specifically the spectrophotometer...
What about it do you have trouble understanding? everything you need is there
edit: after close reading it a bit, it seems like it's a guide to test the mg/ml of oxy
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There's really not much to decipher there, you would need a well equiped lab to do some of that stuff, specifically the spectrophotometer...
What about it do you have trouble understanding? everything you need is there
i seems to me to be the correct means of producing oxymetholone - am i right?
thats all i need to know.
what i don't understand now, i will learn over the next few months.
thanks
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I'm not completly sure that is the whole procedure, that stuff is confusing even for me, but I think it is the right stuff
edit: to put the procedure there in more plain english basicly what they're doing is making a custom TLC plate where they have 2 solutions running up it... Then they scrape those two lines off and then they put them in two diffrent solutions and mix some of those two with a third solution and they you vacuum dry it together with phosphorus pentaoxide for 4 hours before you use the powder
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I'm not completly sure that is the whole procedure, that stuff is confusing even for me, but I think it is the right stuff
Whats the cost to produce 30 pills of eca (100/200/200) , always been trying to find out
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I'm not completly sure that is the whole procedure, that stuff is confusing even for me, but I think it is the right stuff
thanks for the info, it would be easier if i had the patent, but i found it in a pharmacopoeia, so it should be the correct way.
this chemistry stuff is hard :D
good luck with your studies.
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benz, no idea to be honest, I'm on my first year so I'm still just learning the basics here...
And thanks fatpanda :)
btw i edited my other post
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thanks for the info, it would be easier if i had the patent, but i found it in a pharmacopoeia, so it should be the correct way.
this chemistry stuff is hard :D
good luck with your studies.
its not hard when you have access to the tests before you take them :)
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its not hard when you have access to the tests before you take them :)
i'm not sitting a course, just learning at home as a hobby.
have all the instructions i need to make every drug a bodybuilder needs and more, just need the knowhow, chemicals and some lab equipment. 8)
its probably easier just to buy the gear, but i have an unhealthy curiosity about this stuff.
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making gear from scratch is impossible for the average joe(the esterification process can and will kill you without the right equipment) unless you have access to some of the more expensive lab gear... The university lab here is pretty bulky and I could easily make stuff here, but I would never get the green light to do it :p
Easier to just order chinese powders(probably underdosed or fake most of the time) and dissolve it in sterile oil and ba/bb
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making gear from scratch is impossible for the average joe(the esterification process can and will kill you without the right equipment) unless you have access to some of the more expensive lab gear... The university lab here is pretty bulky and I could easily make stuff here, but I would never get the green light to do it :p
Easier to just order chinese powders(probably underdosed or fake most of the time) and dissolve it in sterile oil and ba/bb
you are probably right, but it annoys me that you do not know with 100% accuracy what you are buying.
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Syntex was the company to come out with Anadrol. They were bought up by Roche a while back.
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hes an expert, watch your mouth convict ::)
Shit, candy is showing just how much he doesn't know in this thread. ;D
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Procedure— Proceed as directed for Procedure under 'Single-steroid Assay 511' (see below), using a solvent system consisting of a mixture of benzene and alcohol (98:2), through the fourth sentence of the second paragraph under Procedure. Then centrifuge the tubes for 5 minutes, and determine the absorbances of the supernatants in 1-cm cells at the wavelength of maximum absorbance at about 315 nm, with a suitable spectrophotometer, against the blank. [NOTE—Use 0.01 N alcoholic sodium hydroxide, rather than alcohol, to elute the silica gel bands.] Calculate the quantity, in mg, of C21H32O3 in the portion of Oxymetholone taken by the formula:
10C(AU / AS), in which C is the concentration, in mg per mL, of USP Oxymetholone RS (see below) in the Standard preparation, and AU and AS are the absorbances of the solutions from the Assay preparation and the Standard preparation, respectively.
single-steroid assay 511 - In the following procedure, the steroid to be assayed is separated from related foreign steroids and excipients by thin-layer chromatography and determined following recovery from the chromatogram.
Preparation of the Plate— Prepare a slurry from 30 g of chromatographic silica gel with a suitable fluorescing substance by the gradual addition, with mixing, of about 65 mL of a mixture of water and alcohol (5:2). Transfer the slurry to a clean, 20- × 20-cm plate, spread to make a uniform layer 250 µm thick, and allow to dry at room temperature for 15 minutes. Heat the plate at 105 for 1 hour, and store in a desiccator.
Solvent A— Mix methylene chloride with methanol (180:16).
Solvent B— Mix chloroform with acetone (4:1).
Standard Preparation— Dissolve in a mixture of equal volumes of chloroform and alcohol a suitable quantity of the USP Reference Standard specified in the individual monograph, previously dried as directed (see USP Reference Standards 11) and accurately weighed, to obtain a solution having a known concentration of about 2 mg per mL.
Assay Preparation— Prepare as directed in the individual monograph.
Procedure— Divide the area of the chromatographic plate into three equal sections, the left and right sections to be used for the Assay Preparation and the Standard Preparation, respectively, and the center section for the blank. Apply 200 µL each of the Assay Preparation and the Standard Preparation as streaks 2.5 cm from the bottom of the appropriate section of the plate. Dry the solution as it is being applied, with the aid of a stream of air. Using the Solvent specified in the individual monograph, develop the chromatogram in a suitable chamber, previously equilibrated and lined with absorbent paper, until the solvent front has moved 15 cm above the initial streaks.
Remove the plate, evaporate the solvent, and locate the principal band occupied by the Standard Preparation by viewing under UV light. Mark this band, as well as corresponding bands in the Assay Preparation and blank sections of the plate. Remove the silica gel from each band separately, either by scraping onto glazed weighing papers or by using a suitable vacuum collecting device, and transfer it to a glass-stoppered, 50-mL centrifuge tube. To each tube add 25.0 mL of alcohol, and shake for not less than 2 minutes. Centrifuge the tubes for 5 minutes, pipet 20 mL of the supernatant from each tube into a glass-stoppered, 50-mL conical flask, add 2.0 mL of a solution prepared by dissolving 50 mg of blue tetrazolium in 10 mL of methanol, and mix. Proceed as directed for Procedure under Assay for Steroids 351, beginning with “Then to each flask.”
USP Oxymetholone RS— Dry portion in vacuum over phosphorus pentoxide for 4 hours before using. Keep container tightly closed.
is this what i'm after ?
To me, this reads as a portion of the lab proceedure to identify oxymetholone. It also reads as if there are parts missing. The last paragraph is somewhat disjointed too. Do you have the rest? Procedure under Assay for Steroids 351?
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no thats incorrect. how i explained it to you is accurate.
hahaha funny bastard. ;D
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Vet at first I thought that too, but without the last part it's kinda hard to know, cause what they are doing could be used to make it I guess, just a very wierd way to do it imo... I'm not really into steroid production
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Shit, candy is showing just how much he doesn't know in this thread. ;D
no doubt vet, no doubt. Be sure to save this thread in your harddrive, such opportunities to learn come once in a year and its free! ::) ::) ::) ::)
hahaha funny bastard. ;D
:)
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To me, this reads as a portion of the lab proceedure to identify oxymetholone. It also reads as if there are parts missing. The last paragraph is somewhat disjointed too. Do you have the rest? Procedure under Assay for Steroids 351?
i thought that too at first, its very difficult to work out. thats why i was after the patent. :-\
heres the steroid assay 351 - The following procedure is applicable for determination of those Pharmacopeial steroids that possess reducing functional groups such as -ketols.
Standard Preparation— Dissolve in alcohol a suitable quantity of the USP Reference Standard specified in the individual monograph, previously dried under the conditions specified in the individual monograph and accurately weighed, and dilute quantitatively and stepwise with alcohol to obtain a solution having a concentration of about 10 µg per mL. Pipet 20 mL of this solution into a glass-stoppered, 50-mL conical flask.
Assay Preparation— Prepare as directed in the individual monograph.
Procedure— To each of the two flasks containing the Assay Preparation and the Standard Preparation, respectively, and to a similar flask containing 20.0 mL of alcohol to serve as the blank, add 2.0 mL of a solution prepared by dissolving 50 mg of blue tetrazolium in 10 mL of methanol, and mix. Then to each flask add 2.0 mL of a mixture of alcohol and tetramethylammonium hydroxide TS (9:1), mix, and allow to stand in the dark for 90 minutes. Without delay, concomitantly determine the absorbances of the solutions from the Assay Preparation and the Standard Preparation at about 525 nm, with a suitable spectrophotometer, against the blank. Calculate the result by the formula given in the individual monograph, in which C is the concentration, in µg per mL, of the Reference Standard in the Standard Preparation; and AU and AS are the absorbances of the solutions from the Assay Preparation and the Standard Preparation, respectively.
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thats definately just a identification procedure then
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thats definately just a identification procedure then
>:( :( damn.
i'll have to keep looking then. :'(
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thats definately just a identification procedure then
I agree. And if it was a manufacturing proceedure, the yield would be ridicoulously small.